New Disease Reports (2014) 29, 24. [http://dx.doi.org/10.5197/j.2044-0588.2014.029.024]
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First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliensis in the Netherlands

L. Nunes Leite 1,2, E.G. de Haan 3, M. Krijger 1, P. Kastelein 1, P.S. van der Zouwen 1, G.W. van den Bovenkamp 3, N.D. Tebaldi 2 and J.M. van der Wolf 1*

*jan.vanderwolf@wur.nl

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Received: 25 Nov 2013; Published: 08 Jun 2014

Keywords: Multilocus sequence analysis, bacterial disease

Potato blackleg and stem rot are caused by various soft rot coliforms belonging to the genera Pectobacterium and Dickeya (van der Wolf & De Boer, 2007). In Europe, D. solani, D. dianthicola, P. atrosepticum and P. wasabiae are the main causative agents of the diseases. Recently, in the Netherlands, blackleg diseased plants were found in seed lots produced in different parts of the country, which were positive in a PCR assay for P. carotovorum subsp. brasiliensis (Pcb; Duarte et al., 2004) indicating that Pcb had caused the disease. It was estimated that in 2012, Pcb was responsible for approximately 10 percent of blackleg incidence.

Double-layer crystal violet pectate (DL-CVP) medium (Helias et al., 2012) was used to isolate pectinolytic bacteria from the stems of blackleg affected plants. Eleven isolates derived from ten seed lots, positive in the Pcb-specific PCR (Duarte et al., 2004), were characterised with multilocus sequence analysis (MLSA) using sequences of acnA, gapA, icdA, mdh, mtlD, pgi and proA (Panda et al., 2012) (GenBank Accession Nos. KJ576801-KJ576810, KJ596350-KJ596399, KJ608062-KJ608071). The phylogenetic analysis was done by constructing a maximum likelihood tree using sequences of reference strains P. atrosepticum, P. wasabiae, P.c. subsp. carotovorum, P.c. subsp. odororiferum, P. betavasculorum and Dickeya paradisiaca, derived from GenBank, for comparison (Fig. 1). The MLSA confirmed the identity of the Pcb strains. The Dutch strains clustered together in a homogeneous branch, which also showed that these strains were highly similar. Strains were motile, able to reduce sucrose and to grow at 39°C, resistant to erythromycin, and unable to ferment lactose according to the procedures described by Hayman et al. (1998). Two Pcb isolates were tested for virulence in a field experiment in the Netherlands in 2013. Two months after planting vacuum-infiltrated seed tubers, a high percentage of the plants (more than 30%) showed typical symptoms, whereas water-treated plants remained disease-free (Fig. 2). The pathogen was re-isolated from the diseased plants and identified as Pcb using MLSA. This is the first time Pcb is described as a potato blackleg-causing agent in the Netherlands, although a strain of Pcb isolated from potato in the Netherlands has been found in a culture collection before (De Boer et al., 2012). 

Figure1+
Figure 1: Bootstrap consensus tree of concatenated sequences of acnA, gapA, icdA, mdh, mtlD, pgi and proA of strains belonging to Pectobacterium carotovorum subsp. brasiliensis (Pcb), P. c. subsp. carotovorum (Pcc), P. c. subsp. odoriferous (Pco), P. wasabiae (Pw), P. atrosepticum (Pa), P. betavasculorum (Pbv) and D. paradisiacal (Dp). Strains designated with an IPO number are Pcb isolates from diseased potato plants found in the Netherlands in 2012. Bootstrap percentages were calculated with 500 replicates.
Figure 1: Bootstrap consensus tree of concatenated sequences of acnA, gapA, icdA, mdh, mtlD, pgi and proA of strains belonging to Pectobacterium carotovorum subsp. brasiliensis (Pcb), P. c. subsp. carotovorum (Pcc), P. c. subsp. odoriferous (Pco), P. wasabiae (Pw), P. atrosepticum (Pa), P. betavasculorum (Pbv) and D. paradisiacal (Dp). Strains designated with an IPO number are Pcb isolates from diseased potato plants found in the Netherlands in 2012. Bootstrap percentages were calculated with 500 replicates.
Figure2+
Figure 2: Symptoms caused by Dutch isolates of Pectobacterium carotovorum subsp. brasiliensis strain IPO3650 in a field experiment in the Netherlands in 2013. Tubers (variety Kondor) vacuum-infiltrated with water (A) or with Pcb (B-C).
Figure 2: Symptoms caused by Dutch isolates of Pectobacterium carotovorum subsp. brasiliensis strain IPO3650 in a field experiment in the Netherlands in 2013. Tubers (variety Kondor) vacuum-infiltrated with water (A) or with Pcb (B-C).

Acknowledgements

We acknowledge financial support from the Brazilian Program Science without Borders (CNPq) and the Dutch Ministry of Economic Affairs (program BO-06).


References

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To cite this report: Nunes Leite L, de Haan EG, Krijger M, Kastelein P, van der Zouwen PS, van den Bovenkamp GW, Tebaldi ND, van der Wolf JM, 2014. First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliensis in the Netherlands. New Disease Reports 29, 24. [http://dx.doi.org/10.5197/j.2044-0588.2014.029.024]

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